Application Note: Automated Standard and Sample Preparation for Multiple Gas Chromatographic Analyses of Biodiesel
11 April 2011

This application note by Agilent presents an approach to automating the biodiesel standard and sample preparation prior to GC analysis using an automated liquid sampler (ALS). A derivatization protocol for the silyation of glycerol, mono-, and di-glycerides was developed for the 7693A ALS. A second automated protocol was developed for adding an internal standard to biodiesel samples prior to the fatty acid methyl ester (FAME) analysis. The resulting calibrations and GC sample runs of these methods shows excellent comparison to manual preparation methods.