Application Note: Agilent’s Microarray Platform: How High-Fidelity DNA Synthesis Maximizes the Dynamic Range of Gene Expression Measurements
31 Jul 2013
A key factor controlling the dynamic range of signal intensity in microarray experiments is probe sequence fidelity. Agilent’s SurePrint inkjet oligonucleotide synthesis process dramatically increases the sequence fidelity of probe synthesis, resulting in over five orders of magnitude of dynamic range of signal intensity measurements. This application note describes Agilent’s inkjet DNA chemistry, compares it to both conventional and light-based processes, and discusses how probe quality is related to the dynamic range of signal intensity measurements.