Application Note: Whole Transcript Profiling Using SurePrint G3 Exon Microarrays in a Complete Workflow
15 June 2013

Whole transcript expression profiling is an important tool for understanding biological mechanisms and development, classifying tissue and tumor types, and identifying indicators for diagnosis and prognosis of disease. This type of profiling measures the expression of alternatively spliced exons from a given RNA transcript, which leads to the translation of a variety of proteins from a single RNA transcript. Each of these proteins has a potentially different, and even opposing, function in the cell. Profiling the expression level of individual exons thus provides a more complete representation of protein diversity than traditional microarrays, which measure expression at the gene level. In this application note learn about the advantages of the Agilent complete workflow in whole transcript profiling.