Application Note: Use of the Agilent 2100 Bioanalyzer and DNA 500 LabChip in the Analysis of PCR Amplified Mitochondrial DNA
11 February 2013
Sequence analysis of PCR-amplified mitochondrial DNA (mtDNA) is quickly becoming an accepted tool for forensic testing. Establishing that these amplified products possess the proper size, purity, and concentration to be suitable for sequence analysis is a critical step for forensic mtDNA applications. In this application note, the capability of the Agilent 2100 bioanalyzer and DNA 500 LabChip to rapidly and efficiently analyze mtDNA fragments amplified by PCR prior to sequence determination is demonstrated.