Application Note: Rapid qPCR Using Agilent Brilliant III Ultra-Fast SYBR Green QPCR Master Mix
9 January 2013

In this application note, to optimize qPCR cycle time, the performance of Agilent Brilliant III Ultra-Fast SYBR Green QPCR Master Mix was measured while testing two different sets of cycling parameters. These parameters include a shortened denaturation step, and a shortened combined annealing and elongation step. As a result of these tests, it was determined that the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix achieves high performance and reproducibility, with faster cycle times.

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