MicroRNA Analysis of Archival FFPE Samples by Microarray
7 Jul 2013
MicroRNAs (miRNAs) have diagnostic and prognostic potential for various diseases, most notably for cancer. Microarray-based hybridization has proven to be a powerful technique for miRNA profiling. While many studies have focused on fresh-frozen (FF) tissues, other types of samples such as formalin-fixed paraffin-embedded (FFPE) samples are being explored for retrospective analysis. FFPE samples are of significant value because they are frequently the only sources of tissue available from large patient cohorts with comprehensive clinical data and long-term follow-up spanning decades in some cases. The primary challenge of profiling miRNAs from FFPE samples is the extraction of total RNA that appropriately retains the small RNAs. Most methods for extracting RNA from FFPE samples have been optimized for recovery of significantly longer RNAs. In this application note learn about the various RNA extraction methods that work best in combination with the miRNA microarray profiling system using the miRNA Complete Labeling and Hyb Kit.