Application Note: Method Development for Enantiomers Using the Agilent 1200 Infinity Series Method Development Solution
31 July 2014

Method development for chiral compounds requires chiral columns or modifiers; chiral columns are preferred. Chiral columns are available for reversed phase and normal phase chromatography.In this Application Note, chiral columns, which need normal phase solvents, are used. Trifluoracetic acid (TFA) and diethylamine (DEA) are used as modifiers for the analysis of tofisopam and vanillyl-mandelic acid.

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