Application Note: Measurement of Hepcidin-25 in Serum Using the Agilent 6490 Triple Quadrupole LC/MS with iFunnel Technology
31 August 2014

A novel nano HPLC/MS method has been developed for the rapid, robust, reliable, and accurate determination of hepcidin-25 levels in human serum. It relies on straightforward pre-analytical steps that are cost effective, avoiding the need for solid phase extraction. This application note demonstrates how nano HPLC/MS can be used to deliver better reproducibility and a limit of detection (LOD) for hepcidin less than half that of current C-ELISA assays.