Application Note: High-resolution Analysis of Charge Heterogeneity in Monoclonal Antibodies using pH-gradient Cation Exchange Chromatography
19 February 2013

This Application Note describes a high-resolution, pH-based separation of acidic and basic charge variants for monoclonal antibodies using the Agilent 1260 Infinity Bio-inert Quaternary LC System and an Agilent BiomAb PEEK 4.6 × 250 mm, 5-μm ion exchange column that features a unique resin specifically designed for the charge-based separation of mAbs. The robustness of the method for routine analysis was established by validation studies.

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