Application Note: Five Minute Vitamin D Analysis with Online Sample Cleanup
14 February 2014

Sample preparation is generally the bottleneck in a laboratory’s analytical workflow. With online sample cleanup, sample turnaround time, robustness, and data quality are significantly improved for the LC/MS/MS analysis of both vitamin D forms. This application note from Agilent Technologies describes a fast, sensitive, and selective LC/MS/MS method for quantitative analysis of 25-OH Vitamin D2 and D3 in serum and plasma.