Fast and Efficient HILIC Methods for Improved Analysis of Complex Glycan Structures
24 Jul 2014
In this study, a sub-2 μm UHPLC HILIC column with amide chemistry was used for high-throughput glycosylation profiling. Specifically, we profiled 2-AB labeled human IgG and bovine fetuin N-linked glycans using a 1.8 μm, 2.1 x 150 mm column with fluorescence detection. Rapid, sensitive and selective separations were achieved to provide ultra-high resolution of these complex glycans short run times.