DNA Quality Control of Formalin-Fixed Paraffin-Embedded and Fresh-Frozen Tissues Prior to Target-Enrichment and Next Generation Sequencing
3 May 2013
This application note describes the use of on-chip electrophoresis with the Agilent 2100 Bioanalyzer system for quality control of DNA samples from formalin-fixed paraffin-embedded and fresh-frozen tissues prior to and during SureSelect target enrichment in next generation sequencing workflows. Reliable DNA electrophoresis with the 2100 Bioanalyzer system provided smear profiles and details for library statistics, such as peak heights, average smear size, size distribution, and DNA concentration. FFPE DNA samples gave comparable results to DNA from fresh-frozen tissue and control DNA.