Application Note: Determination of Main and Trace Enantiomers in One Run Using the Agilent 1200 Infinity Series HDR-DAD Impurity Analyzer System
26 June 2014

Chiral compounds are present in many pharmaceutical products, and the determination of each enantiomer during drug development and production is mandatory. The Agilent 1200 Infinity Series High Definition Range Diode Array Detector Impurity Analyzer is the ideal solution to determine trace and main enantiomers with HPLC in one run. Typically, the linear range of the HDR-DAD is extended up to 6,000 mAU. Conversely, it is very sensitive and detects peak heights below 1 mAU with high certainty. This Application Note shows that S-propranolol, with a purity of 99.93 %, was observed in the presence of 0.07 % R-propranolol. Only one run was needed to quantitate both compounds and determine the enantiomeric excess (ee) value.

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