Determination of Chlorinated Acidic Herbicides with a Two-Column System and Duel ECD
8 Dec 2012

The compounds listed in EPA Method 515.1 are acidic and include phenols and carboxylic acids. Although phenols can usually be determined on non-polar fusion silica columns, carboxylic acids require derivatization. This application note from Agilent Technologies describes a derivatization procedure which utilizes Methyl-8 (dimethylformamide dimethyldiacetal) instead of diazomethane suggested in the EPA method. This reagent is simpler to use and less hazardous than diazomethane. Following derivatization, the pesticides were separated on two columns for simultaneous analysis and confirmation.

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