Application Note: Automation of Stratagene Absolutely RNA 96 Microprep Kit with the Bravo Automated Liquid Handling Platform
30 April 2013
This application note presents a new method for the automation of RNA isolation in a 96-well format using the Bravo Automated Liquid Handling Platform and Absolutely RNA 96 Microprep Kit from Agilent Technologies. Using Agilent’s VWorks Automation Control software, a script was written for the Bravo Platform to aspirate, dispense, mix, and transfer sample from a source 96-well tissue culture plate to the binding and collection plates of the Absolutely RNA 96 kit as well as to automate sample wash steps using an accessory vacuum filtration unit on the Bravo Platform. RNA quality was assessed on the Agilent BioAnalyzer 2100.