Automated High-Throughput Analysis of Proteins and Peptides in Blood-Based Matrices Using Immunoaffinity Purification and Ultrafast SPE/MS/MS
27 November 2012
Measuring biomarkers in plasma is challenging due to the complexity of the matrix. Currently, immunoaffinity purification followed by LC/MS/MS is often employed to isolate and quantify proteins or peptides. However, both steps are limited by inefficiency: immunoaffinity is labor intensive, while LC/MS/MS analysis requires minutes per sample. Automation of the immunoaffinity purification step, allowing for simultaneous preparation of 96 samples, followed by online SPE/MS/MS analysis at a rate of seconds per sample could greatly improve the throughput of this workflow. In this application note from Agilent Technologies, such a method was tested to analyze both proteins and peptides from blood-based matrices.