HT Analysis of Epigenetic Targets
3 Jan 2012

Studying the activity of such enzymes that alter gene expression through modification,in a highthroughput manner has proved challenging due to the relatively small molecular alterations as well as the possibility of sequential modifications leading to multiple analytes of interest. This application note describes high-throughput bioassays for the p300 protein, which can serially acetylate multiple lysine residues on the C-terminus of the p53 protein by RapidFire/MS/MS and whole histone proteins by

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