Application Note: A Novel, Substrate-Agnostic Class III HDAC Activity Assay
19 January 2012

Learn about a flexible, homogenous, no–wash assay for quantifying sirtuin activity in this application note. The SIRTainty™ Class lll HDAC Assay is coupled to nicotinamidase, which catalyzes breakdown of nicotinamide generated upon cleavage of NAD+ during sirtuin–mediated deacetylation of a substrate. Thus, the SIRTainty™ assay provides a direct assessment of the activity of class III HDAC enzymes.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me