Application Note: A Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery Using HPLC-Chip/MS-Enabled ESI-TOF MS
15 January 2007
LC/MS-based workflows play an important role in the field of biomarker discovery and validation. Classical shotgun proteomics approaches relying on data-dependent acquisition are generally acknowledged to provide confident identification of only a subset of the actual proteins present in the sample. This application note explores a protein profiling approach combined with differential analysis to highlight and identify putative biomarkers.