Application Note: Research Using Rapid Assessment of AML Genes for Mutation Detection
10 Apr 2014
Targeted sequencing using the Ion AmpliSeq™ Library kit combined with the Ion PGM™ sequencing instrument is a fast and effective research method to identify genetic variants in tumor samples. This application note describes the performance of a new targeted primer panel for amplification of genes involved in Acute Myeloid Leukemia (AML). The panel covers 19 genes characterized using 237 specific primer pairs in two highly multiplexed PCRs.