Application Note: Peptide Mapping of Glycoprotein Erythropoietin by HILIC LC/MS and RP-LC/MS
13 May 2013

Peptide mapping is an important technique for the comprehensive characterization of protein biotherapeutics. Reversed-phase (RP) UHPLC/HPLC is routinely used, but if the digest contains hydrophilic peptides, valuable information can be missed. This application note demonstrates peptide mapping of digested glycoprotein erythropoietin (EPO) using HILIC chromatography as a complementary approach to RP peptide analysis.