Application Note: Monoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing on the Agilent 7100 Capillary Electrophoresis System
10 January 2013

Due to importance of monoclonal antibodies (mAb) as therapeutic agents, there is a growing demand for high resolution analytical methods to characterize these complex molecules. This application note describes the performance of the high resolution capillary isoelectric focusing (cIEF) method for mAb charge isoform analysis on the Agilent 7100 Capillary Electrophoresis (CE) system. The results of an intermediate precision study employing four different CE instruments are presented, with results showing that mAb charge heterogeneity analysis can be performed reliably and with high precision on the Agilent 7100 CE System.

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