Application Note: Gene Expression Microarray Analysis of Archival FFPE Samples
10 May 2011
In this application note by Agilent Technologies, an FFPE sample protocol is developed and optimised for use with Agilent gene expression microarrays. To test the effectiveness of this protocol, total RNA was extracted from a quadruplicate set of colon tumour (adenocarcinoma), and matched normal colon FFPE and fresh frozen tissues. RNA purity and quality were determined, and the RNA was amplified using a system in which a reverse transcription-generated cDNA library is subjected to limited PCR amplification. The resulting cDNA targets were chemically labelled, analysed and compared.