Differentiation of Human Induced Pluripotent Stem Cells to Neural Progenitor Cells and Neurons on Microfluidic Chips
19 Aug 2013

Cell fate determination and cell-cell signaling in vivo involve multiple genes, extracellular factors, and intracellular signaling pathways. Finding the right combinations for in vitro cell differentiation is invaluable for studying fundamental cell biology questions and for developing cell-based therapies. Microfluidic technologies allow for precise control of the microenvironment of cells, facilitate studies of multi-factorial combinations, and enable development of robust, reproducible, and chemically-defined cell culture protocols. This poster presents a prototype microfluidic chip and an automated instrument that can culture cells on chip for extended periods of time and deliver multiple user-defined combinations of different factors to cells.

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