Application Note: Simultaneous Detection and Quantification of Δ9THC, 11-OH-Δ9THC and Δ9THC-COOH in Whole Blood by GC Tandem Quadrupole MS
15 June 2012

A sensitive and reliable method for cannabinoids detection in human whole blood is presented here. The procedure involves extraction of a 1mL specimen acidified with 0.1mL of 10% acetic acid into hexane/ethyl acetate in the presence of deuterated standards. After evaporation, the drug and its metabolites were derivatized by methylation.

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