Rapid Method Development for 18 PAH Compounds with an Agilent RRHD Eclipse PAH column
3 Oct 2011
This application note shows that a fast screening of gradient times with 18 PAH compounds is possible within the 1,200 bar pressure limits of both an Agilent Zorbax RRHD Eclipse PAH column and an Agilent 1290 Infinity UHPLC. An acetonitrile/water gradient was used; as this is the typical mobile phase used for many standard PAH analyses, such as EPA 610 and EPA 8330. Resolution and conditional peak capacity were calculated and used to evaluate the various gradients.