Application Note: Quantification of Mammalian gDNA
11 January 2018

This application note describes how to use the DNA mammalian application on the Lunatic systems. This Unmix application is used to analyze the UV/ Vis spectral shape of the sample to isolate the fraction of the molecule of interest from co-absorbing entities contributing to the total UV/Vis absorption spectrum. This applies to genomic DNA (gDNA) from different mammalian sample sources (human or animal blood, saliva, tissue or cell line...) that are extracted using different extraction methods or commercial kits for DNA isolation.