Precise calculations on rapid kinetics in Photoactivation
23 June 2008
Measuring rapid kinetics of proteins in living cells requires microscope set-ups that enable fast and accurate measurements. To obtain precise kinetic data from FRAP (fluorescence recovery after photobleaching) or photoactivation (PA) experiments it is important to have an easily controllable system for stimulation of a specific region and subsequent imaging. This application note demonstrates how to precisely measure off-rates using a cytosolic photoactivatable probe that binds to endosomal membranes.