Method Development for Size-Exclusion Chromatography of Monoclonal Antibodies and Higher Order Aggregates
2 Apr 2013

Size-exclusion chromatography continues to be a standard technique for the analysis of monoclonal antibodies and their aggregates. However, as in any SEC method, a thorough evaluation needs to be performed to develop an optimum separation. While HP-SEC can be time consuming, the use of UP-SEC allows method optimization to be predicted in less time with a high level of efficiency and higher degree of confidence.

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