Live Cell Kinetics Assay Utilizing the ImageXpress Micro System
31 Oct 2012
In this application note the ability of the ImageXpress® Micro System from Molecular devices, equipped with the Environmental Control and Fluidics option module, to successfully execute a kinetic Transfluor® Cell-Based GPCR Assay is described. The MetaXpress® Software kinetic analysis journal clearly identified cells and measured pits and vesicles formed by the β-arrestin-GFP translocation to activated GPCRs in the Transfluor Assay. The results demonstrate that the ImageXpress Micro System equipped with the Environmental Control and Fluidics Module in conjunction with the Transfluor Assay is a robust tool in conducting kinetics experiments in screening GPCR modulators.