Application Note: Large Insertions: Two Simple Steps Using QuikChange II Site-Directed Mutagenesis Kits
12 February 2013
Insertion and deletion studies offer the ability to map discrete functional areas of genes, to modify vectors, to introduce affinity tags, and to correct frame shifts. However, inserting or deleting large regions of DNA at specific locations can be problematic, as it typically relies on rare or non-existent restriction sites or multiple-step PCR protocols that are time consuming and introduce unwanted errors. This Application Note describes a modified method to introduce large insertions in plasmids in two simple steps using the QuikChange II Site-Directed Mutagenesis kit.