Identification and Functionality of Adult Mouse Hematopoietic Stem Cell Side Populations
21 Jun 2010
This application note demonstrates a protocol for the isolation of HSCs using a combination of cell surface markers that are unique to HSCs, in combination with Hoechst dye efflux and use of the 375-nm near UV laser option on the BD FACSAria II cell sorter. The data demonstrates that the SP cells are phenotypically c-kit+Sca-1+Lin– (KSL), showing that this strategy can be used for the isolation and enrichment of a rare and homogeneous population of potential LT-HSCs that have the ability to differentiate into hematopoietic cell lineages (CFU-G, CFU-GM, and CFU-M).
