Application Note: Human Embryonic Stem Cells Grow on Feeder-Free and Chemically Modified Plastic Plates
13 March 2011

In this application note hESC TW1 are cultured on a chemically modified plastic plate (Corning® Synthemax Surface) with mTeSR medium (a serum-free medium, StemCell Technologies, Inc.) to establish a new feeder-free culture system. Alkaline phosphatase (AP) staining, immunofluorescence staining and flow cytometry is used to analyse hESC TW1 cells for AP activity, typical pluripotent markers and the ability to differentiate into the three germ layers in vitro.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me