High Resolution Melt Parameter Considerations for Optimal Data Resolution
20 May 2011
High resolution melt (HRM) analysis can be used for applications including single nucleotide polymorphism (SNP) analysis, mutation scanning, DNA methylation analysis and species identification. Melt curves are routinely used to analyse the purity of reaction products following real-time qPCR. In this application note, Bio-Rad discuss the analysis steps performed by HRM software to identify thermal profile differences and examine the effect of assay optimisation techniques, temperature increments, and instrument selection on the ability to distinguish different genotypes using HRM analysis.