Application Note: Future-proofing the Biopharmaceutical QC Laboratory: Using the ACQUITY UPLC H-Class Bio System for HPLC Peptide Mapping
15 February 2016
This application note demonstrates a procedure for transferring a peptide mapping method from an HPLC quaternary system to the ACQUITY UPLC® H-Class Bio System. Peptide mapping represents a typical assay for QC labs dealing with routine analyses of large molecule therapeutics. Peptide separation is a considerable challenge in a QC environment due to the numerous parameters that can influence retention time and selectivity. Consequently, adoption of new separation technology such as UPLC can be a challenge in the QC environment due to the qualification requirements for new instruments together with the need to establish and validate new methods for UPLC instrumentation.