Flow-Injection SPR: Sensitive Determinations of Heavy Metal Ions
22 January 2010
As metal ions are complexed by the apo-metallothionein (apo-MT) molecules, the resultant protein conformational changes can be sensitively measured by SPR. The extent of the conformational changes (or thickness variation of the immobilized proteins) is proportional to the amount of metal ions in the sample solution. This method yielded detection levels for Cd2+ and Hg2+ at 0.1 µM and 5 µM, respectively.