Application Note: Development of Fully Scalable Reporter Gene Assays for Studying Transcriptional Regulation and Receptor Activation using Flow Electroporation
16 Nov 2012
In this scientific poster the MaxCyte STX system is shown to provide a rapid and cost effective alternative to stable cell lines for reporter assays. High levels of transfection efficiency and cell viability result in assay responses that are equivalent to or greater than those of stably transfected cells. In this poster the use of MaxCyte electroporation to develop and scale up a variety of reporter gene assays is demonstrated, including expression of TNFα inducible NF-κb reporters and NFAT reporter for examining receptor transcriptional activation.