Application Note: Automated Small RNA Library Preparation with Reduced Bias and High Detection/Discovery Rates

2 August 2017

Small RNAs are short (18 to 30 nucleotides), non-coding RNA molecules that can inhibit the expression of target genes via post-transcriptional gene silencing, chromatin-dependent gene silencing, and RNA activation. Small RNAs play important roles in diverse cellular processes, and research also indicates small RNAs are involved in the pathogenesis of many diseases. This application note demonstrates the benefit of the formation of small RNA libraries via an automated method and shows how the data is comparable to manually prepared libraries. 

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