A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data>

Application Articles

A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
3 Sep 2012

In this study MCM7 was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines. This study demonstrates that inappropriate conclusions can be drawn from qPCR data if the MIQE guidelines are ignored.