The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
|Storage||Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.|
|Buffer||The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.|
|Flow Cytometry (FC), Immunoprecipitation (IP)|