Novel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released From Proteins and Antibodies
27 Jan 2015

Glycans are widely distributed in biological systems in ‘free state’ and conjugated forms such as glycoproteins, glycolipids, and proteoglycans. This application note demonstrates the development of novel high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) columns for high resolution separation and structural characterization of native and fluorescently labeled N-glycans released from various proteins, including antibodies.

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