Benefits of Using the Pippin Prep for Automated Preparative Gel Electrophoresis
1 Dec 2011
This presentation highlights the benefits of using an automated preparative gel electrophoresis system for the construction of next gen sequencing libraries. The Pippin Prep is designed to improve library quality whilst reducing the amount of DNA needed. Results of the use of the Pippin Prep have shown to result in consistent increased yields, more diverse and unique libraries.
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Pippin Prep DNA Size Selection System
Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.
