The Tip Concentrating Effect is a process that allows the recovery of high concentrations of proteins in a single purification procedure. PhyTip pipette tip columns have been designed to produce very high concentrations of recovered proteins compared to competitive techniques such as spin columns, gravity columns, magnetic beads and FPLC. The Tip Concentrating Effect relies on the ability of PhyTip columns to capture protein on very small column bed volumes. Then proteins are recovered in a highly efficient and effective process. All conditions being equal, with any two different resin bed sizes of PhyTip columns, the smaller resin bed size will produce the highest concentration of recovered protein.